Campus Units
Agronomy, Plant Pathology and Microbiology
Document Type
Article
Publication Version
Published Version
Publication Date
2016
Journal or Book Title
Crop Science
Volume
56
Issue
5
First Page
2287
Last Page
2296
DOI
10.2135/cropsci2015.08.0492
Abstract
Brown stem rot (BSR), caused by the fungus Phialophora gregata f. sp. sojae (Allington & D.W. Chamberlain) W. Gams (syn. Cadophora gregata), causes yield losses up to 38%. Three dominant BSR-resistant genes have been identified: Rbs1, Rbs2, and Rbs3. Additional BSR resistance loci will complement breeding efforts by expanding the soybean [Glycine max (L.) Merr.] genetic base. The objective of this research was to determine if PI 594637, PI 594638B, PI 594650A, and PI 594858B contained novel BSR resistance genes. The accessions were crossed to three genotypes with known BSR resistance genes and populations were developed for allelism studies. A minimum of 60 F2:3 families tracing to individual F2 plants in each population were used, and six seeds from each F2:3 family were tested. Resistant and susceptible controls and parents were also included. The BSR symptoms were assessed under growth chamber conditions 5 wk after inoculation by measuring foliar and stem severities and recovery of P. gregata from stem sections. Allelism tests of F2:3 plants from crosses of PI 594638B, PI 594858B, and PI 594650A with the resistant sources fit a 15:1 ratio, indicating that the resistant gene possessed by each of the PIs was nonallelic to Rbs1, Rbs2, and Rbs3. The three PIs contain at least one novel BSR resistance gene and have the potential to serve as donors to elite germplasm, increasing stability of host resistance to P. gregata. Allelism tests of PI 594637 segregated in a 3:1 ratio and no significant difference was found between PI 594637 and the susceptible controls, indicating that PI 594637 is susceptible to BSR.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
McCabe, Chantal E.; Singh, Asheesh K.; Leandro, Leonor F.; Cianzio, Silvia R.; and Graham, Michelle A., "Identifying New Sources of Resistance to Brown Stem Rot in Soybean" (2016). Agronomy Publications. 344.
https://lib.dr.iastate.edu/agron_pubs/344
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Breeding and Genetics Commons, Plant Pathology Commons
Comments
This article is published as McCabe, Chantal E., Asheesh K. Singh, Leonor F. Leandro, Silvia R. Cianzio, and Michelle A. Graham. "Identifying New Sources of Resistance to Brown Stem Rot in Soybean." Crop Science 56, no. 5 (2016): 2287-2296. doi: 10.2135/cropsci2015.08.0492. Posted with permission.