Campus Units

Agronomy

Document Type

Article

Publication Version

Published Version

Publication Date

3-2018

Journal or Book Title

Plant Science

Volume

268

First Page

30

Last Page

38

DOI

10.1016/j.plantsci.2017.12.004

Abstract

Root system architecture (RSA) is becoming recognized as important for water and nutrient acquisition in plants. This study focuses on finding single nucleotide polymorphisms (SNPs) associated with seedling RSA traits from 300 doubled haploid (DH) lines derived from crosses between Germplasm Enhancement of Maize (GEM) accessions and inbred lines PHB47 and PHZ51. These DH lines were genotyped using 62,077 SNP markers, while root and shoot phenotype data were collected from 14-day old seedlings. Genome-wide association studies (GWAS) were conducted using three models to offset false positives/negatives. Multiple SNPs associated with seedling root traits were detected, some of which were within or linked to gene models that showed expression in seedling roots. Significant trait associations involving the SNP S5_152926936 on Chromosome 5 were detected in all three models, particularly the trait network area. The SNP is within the gene model GRMZM2G021110, which is expressed in roots at seedling stage. SNPs that were significantly associated with seedling root traits, and closely linked to gene models that encode proteins associated with root development were also detected. This study shows that the GEM-DH panel may be a source of allelic diversity for genes controlling seedling root development.

Comments

This article is published as Sanchez, Darlene L., Sisi Liu, Rania Ibrahim, Michael Blanco, and Thomas Lübberstedt. "Genome-wide association studies of doubled haploid exotic introgression lines for root system architecture traits in maize (Zea mays L.)." Plant Science 268 (2018): 30-38. doi: 10.1016/j.plantsci.2017.12.004.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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