Campus Units

Agronomy, Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of, Chemistry, Ames Laboratory

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

8-2016

Journal or Book Title

Plant Breeding

Volume

135

Issue

4

First Page

439

Last Page

445

DOI

10.1111/pbr.12382

Abstract

A new fluorescence-based method for inbred haploid differentiation in maize kernels was developed by utilizing the R1-nj color marker in combination with fluorescence micro-spectroscopy and imaging. Seven inbred lines with varying R1-nj expression were used in this study. The fluorescence response of the diploid kernels at the embryonic dye spot was shown to simultaneously exhibit lower intensity and occur at a higher wavelength than the fluorescence of the dye-lacking haploid embryos. Intensity and area thresholds were applied to fluorescence images to sort the haploids from mixed sample populations, and sorting efficiencies of greater than 80% were achieved in all seven inbred lines (with values greater than 90% for five lines). The potential for high throughput sorting when fluorescence imaging is combined with existing technologies for seed handling as well as high sorting efficiency may make fluorescence a viable and promising alternative to current sorting methods for some inbred lines.

Comments

This is the peer reviewed version of the following article: Boote, Brett W., Daniel J. Freppon, Gerald N. De La Fuente, Thomas Lübberstedt, Basil J. Nikolau, and Emily A. Smith. "Haploid differentiation in maize kernels based on fluorescence imaging." Plant Breeding 135, no. 4 (2016): 439-445, which has been published in final form at doi: 10.1111/pbr.12382. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

Copyright Owner

Blackwell Verlag GmbH

Language

en

File Format

application/pdf

Published Version

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