Campus Units
Agronomy
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
10-2015
Journal or Book Title
Trends in Biotechnology
Volume
33
Issue
10
First Page
611
Last Page
620
DOI
10.1016/j.tibtech.2015.07.005
Abstract
Increasing knowledge of plant genome sequences requires the development of more reliable and efficient genetic approaches for genotype-phenotype validation. Functional identification of plant genes is generally achieved by a combination of creating genetic modifications and observing the according phenotype, which begins with forward-genetic methods represented by random physical and chemical mutagenesis and move towards reverse-genetic tools as targeted genome editing. A major bottleneck is time need to produce modified homozygous genotypes that can actually be used for phenotypic validation. Herein, we comprehensively address and compare available experimental approaches for functional validation of plant genes, and propose haploid strategies to reduce the time needed and cost consumed for establishing gene function.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Copyright Owner
Elsevier Ltd
Copyright Date
2015
Language
en
File Format
application/pdf
Recommended Citation
Shen, Yaou; Pan, Guangtang; and Lubberstedt, Thomas, "Haploid Strategies for Functional Validation of Plant Genes" (2015). Agronomy Publications. 452.
https://lib.dr.iastate.edu/agron_pubs/452
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Genetics Commons, Genomics Commons, Plant Breeding and Genetics Commons
Comments
This is a manuscript of an article published as Shen, Yaou, Guangtang Pan, and Thomas Lübberstedt. "Haploid strategies for functional validation of plant genes." Trends in biotechnology 33, no. 10 (2015): 611-620. doi: 10.1016/j.tibtech.2015.07.005. Posted with permission.