Campus Units

Agronomy

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

10-2015

Journal or Book Title

Trends in Biotechnology

Volume

33

Issue

10

First Page

611

Last Page

620

DOI

10.1016/j.tibtech.2015.07.005

Abstract

Increasing knowledge of plant genome sequences requires the development of more reliable and efficient genetic approaches for genotype-phenotype validation. Functional identification of plant genes is generally achieved by a combination of creating genetic modifications and observing the according phenotype, which begins with forward-genetic methods represented by random physical and chemical mutagenesis and move towards reverse-genetic tools as targeted genome editing. A major bottleneck is time need to produce modified homozygous genotypes that can actually be used for phenotypic validation. Herein, we comprehensively address and compare available experimental approaches for functional validation of plant genes, and propose haploid strategies to reduce the time needed and cost consumed for establishing gene function.

Comments

This is a manuscript of an article published as Shen, Yaou, Guangtang Pan, and Thomas Lübberstedt. "Haploid strategies for functional validation of plant genes." Trends in biotechnology 33, no. 10 (2015): 611-620. doi: 10.1016/j.tibtech.2015.07.005. Posted with permission.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Copyright Owner

Elsevier Ltd

Language

en

File Format

application/pdf

Published Version

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