Campus Units

Agronomy

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

2-2013

Journal or Book Title

Plant Physiology

Volume

161

First Page

571

Last Page

582

DOI

10.1104/pp.112.207282

Abstract

Whole-genome sequences established for model and major crop species constitute a key resource for advanced genomic research. For outbreeding forage and turf grass species like ryegrasses (Lolium spp.), such resources are yet to be developed. Here, we present a model of the perennial ryegrass (Lolium perenne L.) genome on the basis of conserved synteny to barley (Hordeum vulgare L.) and the model grass genome Brachypodium (Brachypodium distachyon L.), as well as rice (Oryza sativa L.) and sorghum [Sorghum bicolor (L.) Moench]. A transcriptome-based genetic linkage map of perennial ryegrass served as a scaffold to establish the chromosomal arrangement of syntenic genes from model grass species. This scaffold revealed a high degree of synteny and macro-collinearity, and was then utilised to anchor a collection of perennial ryegrass genes in silico to their predicted genome position. This resulted in the unambiguous assignment of 3,315 out of 8,876 previously unmapped genes to the respective chromosomes. In total, the GenomeZipper incorporates 4,035 conserved grass gene loci which were used for the first genomewide sequence divergence analysis between perennial ryegrass, barley, Brachypodium, rice, and sorghum. The perennial ryegrass GenomeZipper is an ordered, information-rich genome scaffold, facilitating map-based cloning and genome assembly in perennial ryegrass and closely related Poaceae species. It also represents a milestone in describing synteny between perennial ryegrass and fully sequenced model grass genomes, thereby increasing our understanding of genome organization and evolution in the most important temperate forage and turf grass species.

Comments

This is a manuscript of an article published as Pfeifer, Matthias, Mihaela Martis, Torben Asp, Klaus FX Mayer, Thomas Lübberstedt, Stephen Byrne, Ursula Frei, and Bruno Studer. "The perennial ryegrass GenomeZipper: targeted use of genome resources for comparative grass genomics." Plant physiology 161, no. 2 (2013): 571-582. doi: 10.1104/pp.112.207282. Copyright American Society of Plant Biologists. Posted with permission.

Copyright Owner

American Society of Plant Biologists

Language

en

File Format

application/pdf

Published Version

Share

COinS