Campus Units
Agronomy
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
12-2006
Journal or Book Title
Crop Management
Volume
5
Issue
1
DOI
10.1094/CM-2006-0414-01-RS
Abstract
Frost damage in seed corn causes a significant decrease in seed quality. The tetrazolium (TZ) test has been used to identify types of physiological damage in several plant species, including frost damage in corn. Quantifying the amount of seed damaged by frost and the consequent loss of seed vigor are important for making management decisions. The current tetrazolium test procedures provide seed viability information but do not estimate seed vigor. In our project, the tetrazolium staining procedure was modified to allow separation of viable seeds into vigor categories. Vigor categories were defined based on Association of Official Seed Analysts (AOSA) recommendations, previous research, and current observations. The results from the tetrazolium test were compared with standard germination and saturated cold tests during the first 6 months of storage. The staining procedure aided in visual identification of frost damage. Although a strong correlation was found between seed viability as determined by the tetrazolium test, the standard germination test, and the saturated cold test, sorting seeds into vigor categories in the tetrazolium test did not improve the correlation between the tetrazolium test and the saturated cold test.
Copyright Owner
Plant Management Network
Copyright Date
2006
Language
en
File Format
application/pdf
Recommended Citation
DeVries, Mindy and Goggi, A. Susana, "Determining the Extent of Frost Damage in Maize Seed Using the Tetrazolium Test" (2006). Agronomy Publications. 493.
https://lib.dr.iastate.edu/agron_pubs/493
Included in
Agricultural Science Commons, Agronomy and Crop Sciences Commons, Plant Breeding and Genetics Commons
Comments
This is a manuscript of an article published as DeVries, Mindy, and A. Susana Goggi. "Determining the extent of frost damage in maize seed using the tetrazolium test." Crop Management 5, no. 1 (2006). doi: 10.1094/CM-2006-0414-01-RS. Posted wtih permission.