Campus Units

Agronomy

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

11-21-2019

Journal or Book Title

Plant Science

DOI

10.1016/j.plantsci.2019.110337

Abstract

In vivo doubled haploid (DH) technology is widely used in commercial maize (Zea mays L.) breeding. Haploid genome doubling is a critical step in DH breeding. In this study, inbred lines GF1 (0.65), GF3(0.29), and GF5 (0) with high, moderate, and poor spontaneous haploid genome doubling (SHGD), respectively, were selected to develop mapping populations for SHGD. Three QTL, qshgd1, qshgd2, and qshgd3, related to SHGD were identified by selective genotyping. With the exception of qshgd3, the source of haploid genome doubling alleles were derived from GF1. Furthermore, RNA-Seq was conducted to identify putative candidate genes between GF1 and GF5 within the qshgd1 region. A differentially expressed formin-like protein 5 transcript was identified within the qshgd1 region.

Comments

This is a manuscript of an article published as Ren, Jiaojiao, Nicholas Boerman, Ruixiang Liu, Penghao Wu, Benjamin Trampe, Kimberly Vanous, Ursula K. Frei, Shaojiang Chen, and Thomas Lübberstedt. "Mapping of QTL and Identification of Candidate Genes Conferring Spontaneous Haploid Genome Doubling in Maize (Zea mays L.)." Plant Science (2019). doi: 10.1016/j.plantsci.2019.110337. Posted with permission.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Copyright Owner

Elsevier B.V.

Language

en

File Format

application/pdf

Available for download on Saturday, November 21, 2020

Published Version

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