Campus Units
Agronomy
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
2019
Journal or Book Title
The Crop Journal
DOI
10.1016/j.cj.2019.11.004
Abstract
Root system architecture (RSA) contributes to nitrogen (N) uptake and utilization in maize. In this study, a germplasm enhancement of maize double haploid population of 226 lines genotyped with 61,634 SNPs was used to investigate the genetic basis of RSA under two N levels using a genome-wide association study (GWAS). GLM + PCA, FarmCPU, and MLM models were utilized to balance false positives and false negatives. In total, 33 and 51 significant SNP-trait associations were detected under high and low N conditions, respectively. Under high N, SNP S9_2483543 was detected by all models. Linkage disequilibrium (LD) regions of some SNPs overlapped with the intervals of QTL for RSA and N response that were detected in previous studies. In particular, several known genes, Rtcs, Rtcl, Rtcl, and Ms44, were located in the LD regions of S1_9992325, S9_151726472, S9_154381179, and S4_197073985, respectively. Among the candidate genes identified by this study, GRMZM2G139811, GRMZM2G314898, GRMZM2G054050, GRMZM2G173682, GRMZM2G470914, GRMZM2G462325, GRMZM2G416184, and GRMZM2G064302 were involved in seedling, seed, and root system development or N metabolism in Arabidopsis or rice. The markers identified in this study can be used for marker-assisted selection of RSA traits to improve nitrogen use efficiency in maize breeding, and the candidate genes will contribute to further understanding of the genetic basis of RSA under diverse N conditions.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Copyright Owner
Crop Science Society of China and Institute of Crop Science, CAAS
Copyright Date
2019
Language
en
File Format
application/pdf
Recommended Citation
Ma, Langlang; Qing, Chunyan; Frei, Ursula; Shen, Yaou; and Lubberstedt, Thomas, "Association mapping for root system architecture traits under two nitrogen conditions in germplasm enhancement of maize doubled haploid lines" (2019). Agronomy Publications. 614.
https://lib.dr.iastate.edu/agron_pubs/614
Included in
Agriculture Commons, Agronomy and Crop Sciences Commons, Plant Breeding and Genetics Commons
Comments
This is a manuscript of an article published as Ma, Langlang, Chunyan Qing, Ursula Frei, Yaou Shen, and Thomas Lübberstedt. "Association mapping for root system architecture traits under two nitrogen conditions in germplasm enhancement of maize doubled haploid lines." The Crop Journal (2019). doi: 10.1016/j.cj.2019.11.004.