Campus Units
Agronomy
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
12-17-2019
Journal or Book Title
Crop Science
DOI
10.1002/csc2.20021
Abstract
Doubled haploid (DH) lines are used in maize breeding to accelerate the breeding cycle and create homogenous inbred lines in as little as two seasons. These pure inbred lines allow breeders to quickly evaluate new cross combinations. There are two important steps in creating DH lines: 1) generation and selection of haploid progeny, and 2) genome doubling to create fertile, diploid inbreds. Colchicine is widely used to artificially double genomes in haploid plants, which is hazardous, expensive, and time consuming. In this study, three public inbred lines A427, A637, and NK778 were found to have substantial haploid male fertility (HMF). A six-parent full diallel between these three HMF lines and three non-HMF lines was created and HMF was scored. Diallel analysis revealed significant GCA estimates of up to 17% for HMF, as well as significant SCA effects of up to 25%. No significant reciprocal effects were found. HMF is promising to be incorporated into elite maize breeding programs to potentially overcome the need of using colchicine treatments for genome doubling. Colchicine aided doubling success rates varying from almost zero to 30%. HMF has an advantage over artificial genome doubling both in terms of increased success rates and decreased costs for DH line production.
Copyright Owner
The Author(s). Re-use requires permission from the publisher
Copyright Date
2019
Language
en
File Format
application/pdf
Recommended Citation
De La Fuente, Gerald N.; Frei, Ursula K.; Trampe, Benjamin; Ren, Jiaojiao; Bohn, Martin; Yana, Nichole; Verzegnazzi, Anderson; Murray, Seth C.; and Lubberstedt, Thomas, "A diallel analysis of a maize donor population response to In vivo maternal haploid induction II: haploid male fertility" (2019). Agronomy Publications. 616.
https://lib.dr.iastate.edu/agron_pubs/616
Included in
Agriculture Commons, Agronomy and Crop Sciences Commons, Plant Breeding and Genetics Commons
Comments
This is a manuscript of an article published as Gerald, N., Ursula K. Frei, Benjamin Trampe, Jiaojiao Ren, Martin Bohn, Nicole Yana, Anderson Verzegnazzi, Seth C. Murray, and Thomas Lübberstedt. "A diallel analysis of a maize donor population response to In vivo maternal haploid induction II: haploid male fertility." Crop Science (2019). doi: 10.1002/csc2.20021. Posted with permission.