Campus Units

Agronomy

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

12-17-2019

Journal or Book Title

Crop Science

DOI

10.1002/csc2.20021

Abstract

Doubled haploid (DH) lines are used in maize breeding to accelerate the breeding cycle and create homogenous inbred lines in as little as two seasons. These pure inbred lines allow breeders to quickly evaluate new cross combinations. There are two important steps in creating DH lines: 1) generation and selection of haploid progeny, and 2) genome doubling to create fertile, diploid inbreds. Colchicine is widely used to artificially double genomes in haploid plants, which is hazardous, expensive, and time consuming. In this study, three public inbred lines A427, A637, and NK778 were found to have substantial haploid male fertility (HMF). A six-parent full diallel between these three HMF lines and three non-HMF lines was created and HMF was scored. Diallel analysis revealed significant GCA estimates of up to 17% for HMF, as well as significant SCA effects of up to 25%. No significant reciprocal effects were found. HMF is promising to be incorporated into elite maize breeding programs to potentially overcome the need of using colchicine treatments for genome doubling. Colchicine aided doubling success rates varying from almost zero to 30%. HMF has an advantage over artificial genome doubling both in terms of increased success rates and decreased costs for DH line production.

Comments

This is a manuscript of an article published as Gerald, N., Ursula K. Frei, Benjamin Trampe, Jiaojiao Ren, Martin Bohn, Nicole Yana, Anderson Verzegnazzi, Seth C. Murray, and Thomas Lübberstedt. "A diallel analysis of a maize donor population response to In vivo maternal haploid induction II: haploid male fertility." Crop Science (2019). doi: 10.1002/csc2.20021. Posted with permission.

Copyright Owner

The Author(s). Re-use requires permission from the publisher

Language

en

File Format

application/pdf

Available for download on Thursday, December 17, 2020

Published Version

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