Campus Units
Agronomy, Genetics
Document Type
Article
Publication Version
Published Version
Publication Date
9-2003
Journal or Book Title
Proceedings of the National Academy of Sciences
Volume
100
Issue
19
First Page
11127
Last Page
11132
DOI
10.1073/pnas.1836901100
Abstract
TThe B subunit of Escherichia coli heat labile enterotoxin (LT-B) is a potent oral immunogen with potential for use as a vaccine, a carrier molecule to deliver antigens to gut-associated lymphoid tissues, and possibly an adjuvant to make coadministered vaccines more effective. LT-B produced in plants was shown to be functional and immunogenic in animals and humans. In this work, we show that maize-derived LT-B is strongly associated with starch granules in endosperm. Using immunogold labeling/electron microscopy, cell fractionation, and protein analysis techniques, we observed that LT-B protein could be detected both internally and externally in starch granules. This strong association confers an effective copurification of the antigen with the starch fraction of maize kernels, thermostability desirable in maize processing, and resistance to peptic degradation in simulated gastric fluid digests, an important attribute for an orally delivered antigen.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Chikwamba, Rachel Kerina; Scott, Marvin Paul; Mejia, Lorena B.; Mason, Hugh S.; and Wang, Kan, "Localization of a bacterial protein in starch granules of transgenic maize kernels" (2003). Agronomy Publications. 87.
https://lib.dr.iastate.edu/agron_pubs/87
Included in
Agronomy and Crop Sciences Commons, Plant Biology Commons, Plant Breeding and Genetics Commons
Comments
This article is from Proceedings of the National Academy of Sciences 100 (2003): 11127, doi: 10.1073/pnas.1836901100.