Publication Date

2-5-2018

Department

Ames Laboratory; Chemistry; Chemical and Biological Engineering

Campus Units

Ames Laboratory, Chemistry, Chemical and Biological Engineering

Report Number

IS-J 9626

DOI

10.1021/acs.analchem.7b04532

Journal Title

Analytical Chemistry

Volume Number

90

Issue Number

5

First Page

3183

Last Page

3188

Abstract

To establish a new method for tracking the interaction of nanoparticles with chemical cleaving agents, we exploited the optical effects caused by attaching 5–10 nm gold nanoparticles with molecular linkers to large mesoporous silica nanoparticles (MSN). At low levels of gold loading onto MSN, the optical spectra resemble colloidal suspensions of gold. As the gold is removed, by cleaving agents, the MSN revert to the optical spectra typical of bare silica. Time-lapse images of gold-capped MSN stationed in microchannels reveal that the rate of gold release is dependent on the concentration of the cleaving agent. The uncapping process was also monitored successfully for MSN endocytosed by A549 cancer cells, which produce the cleaving agent glutathione. These experiments demonstrate that the optical properties of MSN can be used to directly monitor cleaving kinetics, even in complex cellular settings.

Language

en

Department of Energy Subject Categories

77 NANOSCIENCE AND NANOTECHNOLOGY; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Publisher

Iowa State University Digital Repository, Ames IA (United States)

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