Title

Enhancing the Co-utilization of Biomass-Derived Mixed Sugars by Yeasts

Publication Date

1-22-2019

Department

Ames Laboratory; Chemical and Biological Engineering; Microbiology; NSF Engineering Research Center for Biorenewable Chemicals

Campus Units

Ames Laboratory, Chemical and Biological Engineering, Microbiology, NSF Engineering Research Center for Biorenewable Chemicals

OSTI ID+

1491605

Report Number

IS-J 9840

DOI

10.3389/fmicb.2018.03264

Journal Title

Frontiers in Microbiology

Volume Number

9

First Page

3264

Abstract

Plant biomass is a promising carbon source for producing value-added chemicals, including transportation biofuels, polymer precursors, and various additives. Most engineered microbial hosts and a select group of wild-type species can metabolize mixed sugars including oligosaccharides, hexoses, and pentoses that are hydrolyzed from plant biomass. However, most of these microorganisms consume glucose preferentially to non-glucose sugars through mechanisms generally defined as carbon catabolite repression. The current lack of simultaneous mixed-sugar utilization limits achievable titers, yields, and productivities. Therefore, the development of microbial platforms capable of fermenting mixed sugars simultaneously from biomass hydrolysates is essential for economical industry-scale production, particularly for compounds with marginal profits. This review aims to summarize recent discoveries and breakthroughs in the engineering of yeast cell factories for improved mixed-sugar co-utilization based on various metabolic engineering approaches. Emphasis is placed on enhanced non-glucose utilization, discovery of novel sugar transporters free from glucose repression, native xylose-utilizing microbes, consolidated bioprocessing (CBP), improved cellulase secretion, and creation of microbial consortia for improving mixed-sugar utilization. Perspectives on the future development of biorenewables industry are provided in the end.

DOE Contract Number(s)

AC02-07CH11358

Language

en

Department of Energy Subject Categories

59 BASIC BIOLOGICAL SCIENCES

Publisher

Iowa State University Digital Repository, Ames IA (United States)

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