Publication Date

11-2-2017

Department

Ames Laboratory; Chemistry; Genetics, Development and Cell Biology;

Campus Units

Chemistry, Genetics, Development and Cell Biology, Ames Laboratory

Report Number

IS-J 9494

DOI

10.1038/s41598-017-14949-x

Journal Title

Scientific Reports

Volume Number

7

First Page

14946

Abstract

The zebrafish ( Danio rerio ) has been widely used as a model vertebrate system to study lipid metabolism, the roles of lipids in diseases, and lipid dynamics in embryonic development. Here, we applied high-spatial resolution matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) to map and visualize the three-dimensional spatial distribution of phospholipid classes, phosphatidylcholine (PC), phosphatidylethanolamines (PE), and phosphatidylinositol (PI), in newly fertilized individual zebrafish embryos. This is the first time MALDI-MSI has been applied for three dimensional chemical imaging of a single cell. PC molecular species are present inside the yolk in addition to the blastodisc, while PE and PI species are mostly absent in the yolk. Two-dimensional MSI was also studied for embryos at different cell stages (1-, 2-, 4-, 8-, and 16-cell stage) to investigate the localization changes of some lipids at various cell developmental stages. Four different normalization approaches were compared to find reliable relative quantification in 2D- and 3D- MALDI MSI data sets.

Language

en

Department of Energy Subject Categories

47 OTHER INSTRUMENTATION; 60 APPLIED LIFE SCIENCES

Publisher

Iowa State University Digital Repository, Ames IA (United States)

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