Publication Date

9-19-2018

Department

Ames Laboratory; Chemistry; Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of; Center for Metabolic Biology

Campus Units

Ames Laboratory, Chemistry, Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of, Center for Metabolic Biology

OSTI ID+

1478236

Report Number

IS-J 9768

DOI

10.3389/fpls.2018.01348

Journal Title

Frontiers in Plant Science

Volume Number

9

First Page

1348

Abstract

The visualization of foliage color in plants provides immediate insight into some of the compounds that exist in the leaf. However, many non-colored compounds are also present; their cellular distributions are not readily identifiable optically. In this study we evaluate the applicability of mass spectrometry imaging (MSI) via electrospray laser desorption ionization (ELDI) to reveal the spatial distribution of metabolites. ELDI-MSI is a matrix free, atmospheric pressure ionization method that utilizes a UV laser coupled with supplemental ionization by electrospray. We specifically applied ELDI-MSI to determine the spatial distribution of metabolites in Coleus Henna half leaves that were grown with half-sections either fully illuminated or shaded. We monitored dynamic changes in the spatial distribution of metabolites in response to the change of illumination every 7 days for a 28 day period. A novel source-sink relationship was observed between the 2 halves of the experimental leaf. Furthermore, Coleus Henna leaves present visually recognizable sectors associated with the differential accumulation of flavonoids. Thus, we correlated the effect of differential illumination and presence or absence of flavonoids with metabolic changes revealed by the accumulation of carbohydrates, amino acids, and organic acids. The results show the potential of ELDI-MSI to provide spatial information for a variety of plant metabolites with little sample preparation.

DOE Contract Number(s)

AC02-07CH11358

Language

en

Department of Energy Subject Categories

59 BASIC BIOLOGICAL SCIENCES

Publisher

Iowa State University Digital Repository, Ames IA (United States)

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