Publication Date
9-19-2018
Department
Ames Laboratory; Chemistry; Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of; Center for Metabolic Biology
Campus Units
Ames Laboratory, Chemistry, Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of, Center for Metabolic Biology
OSTI ID+
1478236
Report Number
IS-J 9768
DOI
10.3389/fpls.2018.01348
Journal Title
Frontiers in Plant Science
Volume Number
9
First Page
1348
Abstract
The visualization of foliage color in plants provides immediate insight into some of the compounds that exist in the leaf. However, many non-colored compounds are also present; their cellular distributions are not readily identifiable optically. In this study we evaluate the applicability of mass spectrometry imaging (MSI) via electrospray laser desorption ionization (ELDI) to reveal the spatial distribution of metabolites. ELDI-MSI is a matrix free, atmospheric pressure ionization method that utilizes a UV laser coupled with supplemental ionization by electrospray. We specifically applied ELDI-MSI to determine the spatial distribution of metabolites in Coleus Henna half leaves that were grown with half-sections either fully illuminated or shaded. We monitored dynamic changes in the spatial distribution of metabolites in response to the change of illumination every 7 days for a 28 day period. A novel source-sink relationship was observed between the 2 halves of the experimental leaf. Furthermore, Coleus Henna leaves present visually recognizable sectors associated with the differential accumulation of flavonoids. Thus, we correlated the effect of differential illumination and presence or absence of flavonoids with metabolic changes revealed by the accumulation of carbohydrates, amino acids, and organic acids. The results show the potential of ELDI-MSI to provide spatial information for a variety of plant metabolites with little sample preparation.
DOE Contract Number(s)
AC02-07CH11358
Language
en
Department of Energy Subject Categories
59 BASIC BIOLOGICAL SCIENCES
Publisher
Iowa State University Digital Repository, Ames IA (United States)
