Campus Units
Chemistry, Ames Laboratory
Document Type
Article
Publication Version
Published Version
Publication Date
10-2011
Journal or Book Title
Journal of the American Chemical Society
Volume
133
Issue
46
First Page
18554
Last Page
18557
DOI
10.1021/ja2080168
Abstract
We report a gold nanoparticle (AuNP)-capped mesoporous silica nanoparticle (Au-MSN) platform for intracellular codelivery of an enzyme and a substrate with retention of bioactivity. As a proof-of-concept demonstration, Au-MSNs are shown to release luciferin from the interior pores of MSN upon AuNP uncapping in response to disulfide-reducing antioxidants and codeliver bioactive luciferase from the PEGylated exterior surface of Au-MSN to Hela cells. The effectiveness of luciferase-catalyzed luciferin oxidation and luminescence emission in the presence of intracellular ATP was measured by a luminometer. Overall, the chemical tailorability of the Au-MSN platform to retain enzyme bioactivity, the ability to codeliver enzyme and substrate, and the potential for imaging tumor growth and metastasis afforded by intracellular ATP- and glutathione-dependent bioluminescence make this platform appealing for intracellular controlled catalysis and tumor imaging.
Copyright Owner
American Chemical Society
Copyright Date
2011
Language
en
File Format
application/pdf
Recommended Citation
Sun, Xiaoxing; Zhao, Yannan; Lin, Victor S.-Y.; Slowing, Igor I.; and Trewyn, Brian G., "Luciferase and Luciferin Co-immobilized Mesoporous Silica Nanoparticle Materials for Intracellular Biocatalysis" (2011). Ames Laboratory Publications. 306.
https://lib.dr.iastate.edu/ameslab_pubs/306
Comments
Reprinted (adapted) with permission from Journal of the American Chemical Society 133 (2011): 18554, doi:10.1021/ja2080168. Copyright 2011 American Chemical Society.