Campus Units

Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of, Chemical and Biological Engineering, Materials Science and Engineering, Physics and Astronomy, Ames Laboratory

Document Type

Article

Publication Version

Submitted Manuscript

Publication Date

7-13-2016

Journal or Book Title

Langmuir

Volume

32

Issue

30

First Page

7664

Last Page

7670

DOI

10.1021/acs.langmuir.6b01851

Abstract

Surface-sensitive X-ray scattering and spectroscopy techniques reveal significant adsorption of iron ions and iron-hydroxide (Fe(III)) complexes to a charge-neutral zwitterionic template of phosphatidylcholine (PC). The PC template is formed by a Langmuir monolayer of dipalmitoyl-PC (DPPC) that is spread on the surface of 2 to 40 μM FeCl3 solutions at physiological levels of KCl (100 mM). At 40 μM of Fe(III) as many as ∼3 iron atoms are associated with each PC group. Grazing incidence X-ray diffraction measurements indicate a significant disruption in the in-plane ordering of DPPC molecules upon iron adsorption. The binding of iron-hydroxide complexes to a neutral PC surface is yet another example of nonelectrostatic, presumably covalent bonding to a charge-neutral organic template. The strong binding and the disruption of in-plane lipid structure has biological implications on the integrity of PC-derived lipid membranes, including those based on sphingomyelin.

Comments

This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Langmuir, copyright © American Chemical Society after peer review. To access the final edited and published work see http://pubs.acs.org/doi/10.1021/acs.langmuir.6b01851. Posted with permission.

Copyright Owner

American Chemical Society

Language

en

File Format

application/pdf

Share

COinS