Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of, Chemical and Biological Engineering, Materials Science and Engineering, Physics and Astronomy, Ames Laboratory
Journal or Book Title
Surface-sensitive X-ray scattering and spectroscopy techniques reveal significant adsorption of iron ions and iron-hydroxide (Fe(III)) complexes to a charge-neutral zwitterionic template of phosphatidylcholine (PC). The PC template is formed by a Langmuir monolayer of dipalmitoyl-PC (DPPC) that is spread on the surface of 2 to 40 μM FeCl3 solutions at physiological levels of KCl (100 mM). At 40 μM of Fe(III) as many as ∼3 iron atoms are associated with each PC group. Grazing incidence X-ray diffraction measurements indicate a significant disruption in the in-plane ordering of DPPC molecules upon iron adsorption. The binding of iron-hydroxide complexes to a neutral PC surface is yet another example of nonelectrostatic, presumably covalent bonding to a charge-neutral organic template. The strong binding and the disruption of in-plane lipid structure has biological implications on the integrity of PC-derived lipid membranes, including those based on sphingomyelin.
American Chemical Society
Wang, Wenji; Zhang, Honghu; Feng, Shuren; San Emeterio, Josue; Mallapragada, Surya K.; and Vaknin, David, "Iron Ion and Iron Hydroxide Adsorption to Charge-Neutral Phosphatidylcholine Templates" (2016). Ames Laboratory Publications. 401.