Mycoplasma ovipneumoniae infection is a problem among lambs raised in indoor confinement operations. This study looked at different M. ovipneumoniae culture and identification techniques to determine preferred methods of detection and genomic fingerprinting. The results indicated that SP43+ media and the Hum4 primer AP-PCR system were best for culturing and fingerprinting M. ovipneumoniae. Using these methods will allow for studies on transmission of M. ovipneumoniae in barn raised lambs.