Identification of Swine Salmonella serotypes Using Pulsed-field Gel Electrophoresis of Conserved Xba1 Fragments
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2007-01-01
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Gaul, Stephen
Wedel, Stephanie
Erdman, Matthew
Harris, D.L.
Harris, Isabel
Ferris, Kathleen
Hoffman, Lorraine
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Abstract
Swine Salmonella isolates (n=674) from various locations throughout the United States and Canada were analyzed via pulsed-field gel electrophoresis (PFGE) with Xba1. PFGE subtypes were analyzed by cluster analysis and compared to conventional serotyping results. The analysis showed a correlation of serotype to PFGE subtype. In addition, conserved fragments were identified within the restriction patterns that were unique to each serotype. PFGE using Xba1 restriction provides a screening method for identifying swine Salmonella serotypes.
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Mon Jan 01 00:00:00 UTC 2007