Effect of lipopolysaccharide (LPS) injection and dietary natural source vitamin E (NSVE) levels on RNA expression levels of 14 immune related genes were measured in peripheral blood lymphocytes of broiler. Broilers immunologically responded to injection of LPS (bacterial membrane component) with a pro-inflammatory response of interleukin 6 (IL6) and an innate response of avian beta-defensing 10 (AvBD10) and inducible nitric oxide synthase (iNOS). The genes profiled in this study did not differed only moderately in RNA expression level between the NSVE dietary levels. This study confirmed and expanded our understanding of the network of genes involved in chicken’s immune response to bacterial infection. Newly described AvBD10 RNA expressed in LPS-injected birds emphasizes the importance of innate immunity in defense against bacterial infection.