It was observed that diets with high sulfur and molybdenum decreased markers of copper, selenium, and manganese after 90 d. In a 62-d trace mineral repletion period, steers receiving an injection of Multimin90 had the most rapid improvement of copper and selenium status by d 14, while it took 28 d and 42 d for copper and selenium status to improve in steers supplemented with 150% of national recommended concentrations from either an inorganic and organic blend, or only inorganic trace minerals. Further research is needed to understand the optimal trace mineral supplementation strategy to overcome dietary antagonisms without creating economic loss for producers.