Document Type

Article

Publication Version

Published Version

Publication Date

2010

Journal or Book Title

Journal of Biomedicine and Biotechnology

Volume

2010

First Page

1

Last Page

11

DOI

10.1155/2010/453638

Abstract

The design of oligonucleotide sequences for the detection of gene expression in species with disparate volumes of genome and EST sequence information has been broadly studied. However, a congruous strategy has yet to emerge to allow the design of sensitive and specific gene expression detection probes. This study explores the use of a phylogenomic approach to align transcribed sequences to vertebrate protein sequences for the detection of gene families to design genomewide 70-mer oligonucleotide probe sequences for bovine and porcine. The bovine array contains 23,580 probes that target the transcripts of 16,341 genes, about 72% of the total number of bovine genes. The porcine array contains 19,980 probes targeting 15,204 genes, about 76% of the genes in the Ensembl annotation of the pig genome. An initial experiment using the bovine array demonstrates the specificity and sensitivity of the array.

Comments

This article is from Journal of Biomedicine and Biotechnology 2010 (2010): 453638, doi:10.1155/2010/453638. Posted with permission.

Rights

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Copyright Owner

John R. Garbe, et al.

Language

en

File Format

application/pdf

Share

COinS