Document Type
Article
Publication Version
Published Version
Publication Date
11-2015
Journal or Book Title
PLoS One
Volume
10
Issue
11
First Page
1
Last Page
17
DOI
10.1371/journal. pone.0142570
Abstract
Extraintestinal pathogenic Escherichia coli (ExPEC) has major negative impacts on human and animal health. Recent research suggests food-borne links between human and animal ExPEC diseases with particular concern for poultry contaminated with avian pathogenic E. coli (APEC), the avian ExPEC. APEC is also a very important animal pathogen, causing colibacillosis, one of the world’s most widespread bacterial diseases of poultry. Previous studies showed marked atrophy and lymphocytes depletion in the bursa during APEC infection. Thus, a more comprehensive understanding of the avian bursa response to APEC infection will facilitate genetic selection for disease resistance. Four-week-old commercial male broiler chickens were infected with APEC O1 or given saline as a control. Bursas were collected at 1 and 5 days post-infection (dpi). Based on lesion scores of liver, pericardium and air sacs, infected birds were classified as having mild or severe pathology, representing resistant and susceptible phenotypes, respectively. Twenty-two individual bursa RNA libraries were sequenced, each yielding an average of 27 million single-end, 100-bp reads. There were 2469 novel genes in the total of 16,603 detected. Large numbers of significantly differentially expressed (DE) genes were detected when comparing susceptible and resistant birds at 5 dpi, susceptible and non-infected birds at 5 dpi, and susceptible birds at 5 dpi and 1 dpi. The DE genes were associated with signal transduction, the immune response, cell growth and cell death pathways. These data provide considerable insight into potential mechanisms of resistance to ExPEC infection, thus paving the way to develop strategies for ExPEC prevention and treatment, as well as enhancing innate resistance by genetic selection in animals.
Rights
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Copyright Owner
Hongyan Sun, et al.
Copyright Date
2015
Language
en
File Format
application/pdf
Recommended Citation
Sun, Hongyan; Liu, Peng; Nolan, Lisa K.; and Lamont, Susan J., "Novel Pathways Revealed in Bursa of Fabricius Transcriptome in Response to Extraintestinal Pathogenic Escherichia coli (ExPEC) Infection" (2015). Animal Science Publications. 180.
https://lib.dr.iastate.edu/ans_pubs/180
Included in
Agriculture Commons, Genetics Commons, Poultry or Avian Science Commons, Veterinary Infectious Diseases Commons
Comments
This article is from PLoS One 10 (2015): e0142570, doi:10.1371/journal.pone.0142570. Posted with permission.