Document Type
Article
Publication Version
Published Version
Publication Date
3-2014
Journal or Book Title
Scientific Reports
Volume
4
First Page
1
Last Page
10
DOI
10.1038/srep04299
Abstract
Avian pathogenic Escherichia coli (APEC) are responsible for heavy economic losses in poultry industry. Here we investigate DNA methylome of spleen and identify functional DNA methylation changes related to host response to APEC among groups of non-challenged chickens (NC), challenged with mild (MD) and severe pathology (SV). DNA methylation was enriched in the gene bodies and repeats. Promoter and CGIs are hypomethylated. Integration analysis revealed 22, 87, and 9 genes exhibiting inversely changed DNA methylation and gene expression in NC vs. MD, NC vs. SV, and MD vs. SV, respectively.IL8, IL2RB, and IL1RAPL1 were included. Gene network analysis suggested that besides inflammatory response, other networks and pathways such as organismal injury and abnormalities, cell signaling and molecular transport, are probably related to host response to APEC infection. Moreover, methylation changes in cell cycle processes might contribute to the lesion phenotype differences between MD and SV.
Rights
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Copyright Owner
Haiping Xu, et al
Copyright Date
2014
Language
en
File Format
application/pdf
Recommended Citation
Xu, Haiping; Zhu, Xuenong; Hu, Yongsheng; Li, Zhenhui; Zhang, Xiquan; Nie, Qinghua; Nolan, Lisa K.; and Lamont, Susan J., "DNA Methylome in Spleen of Avian Pathogenic Escherichia coli-Challenged Broilers and Integration with mRNA Expression" (2014). Animal Science Publications. 184.
https://lib.dr.iastate.edu/ans_pubs/184
Comments
This article is from Scientific Reports 4 (2014): 4299, doi:10.1038/srep04299. Posted with permission.