Document Type

Article

Publication Version

Published Version

Publication Date

2003

Journal or Book Title

Genetics Selection Evolution

Volume

35

Issue

Suppl. 1

First Page

S99

Last Page

S111

DOI

10.1051/gse:2003019

Abstract

Several candidate genes were selected, based on their critical roles in the host’s response to intracellular bacteria, to study the genetic control of the chicken response to Salmonella enteritidis (SE). The candidate genes were: inducible nitric oxide synthase (INOS), tumor necrosis factor related apoptosis inducing ligand (TRAIL), transforming growth factor b2 (TGF-b2), transforming growth factor b3 (TGF-b3), and immunoglobulin G light chain (IgL). Responses to pathogenic SE colonization or to SE vaccination were measured in the Iowa Salmonella response resource population (ISRRP). Outbred broiler sires and three diverse, highly inbred dam lines produced 508 F1 progeny, which were evaluated as young chicks for either bacterial load isolated from spleen or cecum contents after pathogenic SE inoculation, or the circulating antibody level after SE vaccination. Fragments of each gene were sequenced from the founder lines of the resource population to identify genomic sequence variation. Single nucleotide polymorphisms (SNP) were identified, then PCR-RFLP techniques were developed to genotype the F1 resource population. Linear mixedmodels were used for statistical analyses. Because the inbred dam lines always contributed one copy of the same allele, the heterozygous sire allele effects could be assessed in the F1 generation. Association analyses revealed significant effects of the sire allele of TRAIL-StyI on the spleen (P < 0:07) and cecum (P < 0:0002) SE bacterial load. Significant effects (P < 0:04) were found on the cecum bacterial load for TGF-b3-BsrI. Varied and moderate association was found for SE vaccine antibody response for all genes. This is the first reported study on the association of SNP in INOS, TRAIL, TGF-b2, TGF-b3, and IgL with the chicken response to SE. Identification of candidate genes to improve the immune response may be useful for marker-assisted selection to enhance disease resistance.

Comments

This article is from Genetics Selection Evolution 35 (2003): S99, doi:10.1051/gse:2003019. Posted with permission.

Rights

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Copyright Owner

Massoud Malek and Susan J. Lamont

Language

en

File Format

application/pdf

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