Document Type

Article

Publication Version

Published Version

Publication Date

1990

Journal or Book Title

Poultry Science

Volume

69

Issue

7

First Page

1195

Last Page

1203

DOI

10.3382/ps.0691195

Abstract

Eight 15I5 B-congenic White Leghorn chicken lines, containing haplotypesB 2 , B 5 , B 12, B 13, B 15 , B 19 , and B 21, were subjected to molecular genotyping with chicken B-F (Class I) and B-L (Class II) major histocompatibility complex (MHC) probes. Genomic DNA was digested with restriction enzymes, hybridized with a Class I or Class II probe, and analyzed for restriction fragment length polymorphisms. Digestion withHindIII or EcoRI yielded no B-L polymorphisms. Digestion with PvuII or BglII and hybridization with a B-L or B-F probe produced polymorphisms that were shared between several haplotypes, although the haplotypes with similar patterns were clustered differently between Class I and Class II probes. The genetic variation seen for B-L and B-F probe hybridization ofPvuII digests was much less than that previously demonstrated for B-Gprobing of PvuII digests of the same lines. Description of MHC Class I and II restriction patterns of the well-characterized 15I5 B-congenic lines will aid in identification of genes important in disease resistance.

Comments

This article is from Poultry Science 69 (1990): 1195, doi:10.3382/ps.0691195.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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