Campus Units
Animal Science, Statistics, Bioinformatics and Computational Biology
Document Type
Article
Publication Version
Published Version
Publication Date
2-1-2010
Journal or Book Title
American Journal of Physiology - Regulatory, Integrative and Comparative Physiology
Volume
298
Issue
2
First Page
R494
Last Page
R507
DOI
10.1152/ajpregu.00632.2009
Abstract
Residual feed intake (RFI) is a measure of feed efficiency, in which low RFI denotes improved feed efficiency. Caloric restriction (CR) is associated with feed efficiency in livestock species and to human health benefits, such as longevity and cancer prevention. We have developed pig lines that differ in RFI, and we are interested in identifying the genes and pathways that underlie feed efficiency. Prepubertal Yorkshire gilts with low RFI (n = 10) or high RFI (n = 10) were fed ad libitum or fed at restricted intake of 80% of maintenance energy requirements for 8 days. We measured serum metabolites and hormones and generated transcriptional profiles of liver and subcutaneous adipose tissue on these animals. Overall, 6,114 genes in fat and 305 genes in liver were differentially expressed (DE) in response to CR, and 311 genes in fat and 147 genes in liver were DE due to RFI differences. Pathway analyses of CR-induced DE genes indicated a dramatic switch to a conservation mode of energy usage by down-regulating lipogenesis and steroidogenesis in both liver and fat. Interestingly, CR altered expression of genes in immune and cell cycle/apoptotic pathways in fat, which may explain part of the CR-driven lifespan enhancement. In silico analysis of transcription factors revealed ESR1 as a putative regulator of the adaptive response to CR, as several targets of ESR1 in our DE fat genes were annotated as cell cycle/apoptosis genes. The lipid metabolic pathway was overrepresented by down-regulated genes due to both CR and low RFI. We propose a common energy conservation mechanism, which may be controlled by PPARA, PPARG, and/or CREB in both CR and feed-efficient pigs.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Lkhagvadorj, Sender; Qu, Long; Cai, Weiguo; Couture, Oliver P.; Barb, C. Richard; Hausman, Gary J.; Nettleton, Dan; Anderson, Lloyd L.; Dekkers, Jack C. M.; and Tuggle, Christopher K., "Gene expression profiling of the short-term adaptive response to acute caloric restriction in liver and adipose tissues of pigs differing in feed efficiency" (2010). Animal Science Publications. 216.
https://lib.dr.iastate.edu/ans_pubs/216
Included in
Agriculture Commons, Animal Sciences Commons, Bioinformatics Commons, Biometry Commons, Computational Biology Commons, Computational Neuroscience Commons
Comments
This article is from American Journal of Physiology - Regulatory, Integrative and Comparative Physiology 298 (2010): R494–R507, doi:10.1152/ajpregu.00632.2009.