Journal or Book Title
Journal of Animal Science
Source and Description of Primers. Primers for the very-long-chain acyl-CoA dehydrogenase (ACADVL) gene were designed from a bovine cDNA sequence (GenBank accession No. U30817) aligned with the human ACADVL gene (GenBank accession No. L46590). The forward primer was 5¢-TTT GGG GAG AAA ATT CAC AAC-3¢ and the reverse primer was 5¢-GCG GCC TCT ATC TGG AAG T-3¢. The amplification product was expected to span from exon 11 to exon 12 of the ACADVL gene. Exonic parts (103 bp) of the pig sequence were 91% identical at the nucleotide level with the human ACADVL sequence. The pig sequence produced here has been submitted to GenBank (accession no. AF022255).
American Society of Animal Science
Kenealy, S.; Larsen, N. J.; Tuggle, Christopher K.; and Rothschild, Max F., "Rapid Communication: The Very-Long-Chain Acyl-CoA Dehydrogenase Gene Maps to Pig Chromosome 12" (1998). Animal Science Publications. 259.