Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

1998

Journal or Book Title

Journal of Animal Science

Volume

76

Issue

10

First Page

2747

Last Page

2748

DOI

/1998.76102747x

Abstract

Source and Description of Primers. The forward primer was developed from a human fibroblast growth factor ( FGF7) cDNA sequence (GenBank accession no. L06243), and the reverse primer was a published primer (Kelley et al., 1992). These primers were used to amplify a 1.3-kb fragment from porcine genomic DNA. This fragment included regions corresponding to exon 2, exon 3, and the intron flanked by these two exons. Sequences were obtained from both ends of the PCR fragment and compared with a human sequence showing 95.9% identity at the amino acid level in a 73-amino acid overlap. Sequences produced in this experiment have been submitted to GenBank (accession no. AF052657).

Comments

This is an article from Journal of Animal Science 76 (1998): 2747, doi:/1998.76102747x. Posted with permission.

Copyright Owner

American Society of Animal Science

Language

en

File Format

application/pdf

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