Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

1999

Journal or Book Title

Journal of Animal Science

Volume

77

Issue

2

First Page

494

Last Page

495

DOI

/1999.772494x

Abstract

Source and Description of Primers. Primers were designed from published sheep epidermal growth factor ( EGF) sequence (GenBank accession no. U36428) and were used to amplify across intron 3 of the EGF gene from porcine genomic DNA. Of sequences produced, 84 bp showed 82.1% identity to sheep EGF exon 3 and 81.0% to human EFG exon 3. For exon 4, the 75 bp showed 82.3% identity to sheep EGF exon 4 and 77.3% identity to human EGF exon 4. These sequences were further used to design pigspecific primers and have been submitted to GenBank (accession nos. AF079768 and AF079769).

Comments

This is an article from Journal of Animal Science 77 (1999): 494, doi:/1999.772494x. Posted with permission.

Copyright Owner

American Society of Animal Science

Language

en

File Format

application/pdf

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