Journal or Book Title
Journal of Animal Science
Source and Description of Primers. Primers were designed from published sheep epidermal growth factor ( EGF) sequence (GenBank accession no. U36428) and were used to amplify across intron 3 of the EGF gene from porcine genomic DNA. Of sequences produced, 84 bp showed 82.1% identity to sheep EGF exon 3 and 81.0% to human EFG exon 3. For exon 4, the 75 bp showed 82.3% identity to sheep EGF exon 4 and 77.3% identity to human EGF exon 4. These sequences were further used to design pigspecific primers and have been submitted to GenBank (accession nos. AF079768 and AF079769).
American Society of Animal Science
Mendez, E. A.; Messer, L. A.; Larsen, N. J.; Robic, A.; and Rothschild, Max F., "Rapid Communication: Epidermal Growth Factor Maps to Pig Chromosome 8" (1999). Animal Science Publications. 264.