Rapid Communication: Assignment of Porcine Serotonin Receptor Subtype 2 Alpha and Endothelin-B Receptor to Chromosome 11 by Linkage Analysis
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The Department of Animal Science originally concerned itself with teaching the selection, breeding, feeding and care of livestock. Today it continues this study of the symbiotic relationship between animals and humans, with practical focuses on agribusiness, science, and animal management.
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The Department of Animal Husbandry was established in 1898. The name of the department was changed to the Department of Animal Science in 1962. The Department of Poultry Science was merged into the department in 1971.
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- Department of Animal Husbandry (1898–1962)
- College of Agricultural and Life Sciences (parent college)
- Department of Poultry Science (merged with, 1971)
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Abstract
Genus and Species. Sus scrofa. Locus. Pig serotonin receptor subtype 2 alpha ( HTR2A) and pig endothelin-B receptor ( EDNRB) . Source and Description of Primers. Heterologous primers for EDNRB were obtained from Leslie Lyons through the CATS project (Lyons et al., 1997) and primers for HTR2A were designed from highly conserved human (M86841) and mouse sequences (X72222). Primers ( EDNRB forward: 5¢-AATTGTTTTAATTTGGGTGGTCTC- 3¢ and EDNRB reverse: 5¢-AGCCACCAGTCTTTAGCTGTC-3¢; HTR2A forward: 5¢-CCCTAGAGAAAAAGCTGCAGA-3¢ and HTR2A reverse: 5¢-GACACGGGCATGACAAGGA-3¢) were used to amplify pig homologous fragments by standard PCR. Pig genomic fragments amplified with heterologous primers were sequenced to confirm homology. An 81% similarity over 125 bp was found in the exon 3 region of the EDNRB gene between human and the new pig sequence-tagged sites ( STS) . A 100% sequence similarity was found for 115 bases of the exon 1 region of HTR2A gene between our STS and a pig HTR2A cDNA sequence (accession no. S78208). Pig-specific primers were designed from the pig STS obtained in this study ( HTR2A forward: 5¢- CCCTAGAGAAAAAGCTG CAGA-3¢ and HTR2A reverse: 5¢-GCAGAGGCCACCGGTA-3¢) to increase the PCR efficiency.
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This is an article from Journal of Animal Science 77 (1999): 795, doi:/1999.773795x. Posted with permission.