Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

1999

Journal or Book Title

Journal of Animal Science

Volume

77

Issue

3

First Page

791

Last Page

792

DOI

/1999.773791x

Abstract

Source and Description of Primers. Primers for the Cocaine- and Amphetamine-Regulated Transcript ( CART) gene were designed from homologous regions of published human and rat CART sequences (Gen- Bank accession nos. U20325 and U10071, respectively). The primers were used to amplify a 468-bp fragment of the porcine CART gene spanning exons 1 and 2. Porcine CART sequences were sequenced from animals of the Meishan and Hampshire breeds (GenBank accession nos. AFO81918 and AFO81919, respectively) and showed 96% exonic nucleotide identity to the corresponding human CART sequence. Additionally, pig-specific primers were designed for use of the pig/rodent somatic cell hybrids to physically map the CART gene.

Comments

This is an article from Journal of Animal Science 77 (1999): 791, doi:/1999.773791x. Posted with permission.

Copyright Owner

American Society of Animal Science

Language

en

File Format

application/pdf

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