Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

1999

Journal or Book Title

Journal of Animal Science

Volume

77

Issue

10

First Page

2857

Last Page

2858

DOI

/1999.77102857x

Abstract

Source and Description of Primers. The initial primers for the PCR were designed based on human DNA sequence (accession no. X92412; Kolmerer et al., 1996). The position of the forward and reverse primers corresponded to exon 3 and exon 5, respectively. These primers are expected to amplify a fragment of 1.93 kb from human DNA. A PCR fragment of 2.1 kb was amplified from porcine genomic DNA. Partial sequences were compared with the human sequence, which showed 90% (1,038/1,159 bp) nucleotide identity. The pig sequences (AF124849-50) were then used to design additional primers that amplified a 1.8-kb fragment of pig TTN and were used both for physical mapping using the somatic cell hybrid panel (SCHP) and for PCR-RFLP analysis.

Comments

This is an article from Journal of Animal Science 77 (1999): 2857, doi:/1999.77102857x. Posted with permission.

Copyright Owner

American Society of Animal Science

Language

en

File Format

application/pdf

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