Campus Units
Animal Science
Document Type
Article
Publication Version
Published Version
Publication Date
1999
Journal or Book Title
Journal of Animal Science
Volume
77
Issue
10
First Page
2857
Last Page
2858
DOI
/1999.77102857x
Abstract
Source and Description of Primers. The initial primers for the PCR were designed based on human DNA sequence (accession no. X92412; Kolmerer et al., 1996). The position of the forward and reverse primers corresponded to exon 3 and exon 5, respectively. These primers are expected to amplify a fragment of 1.93 kb from human DNA. A PCR fragment of 2.1 kb was amplified from porcine genomic DNA. Partial sequences were compared with the human sequence, which showed 90% (1,038/1,159 bp) nucleotide identity. The pig sequences (AF124849-50) were then used to design additional primers that amplified a 1.8-kb fragment of pig TTN and were used both for physical mapping using the somatic cell hybrid panel (SCHP) and for PCR-RFLP analysis.
Copyright Owner
American Society of Animal Science
Copyright Date
1999
Language
en
File Format
application/pdf
Recommended Citation
Bertani, G. R.; Larsen, N. J.; Marklund, S.; Hu, Zhiliang; and Rothschild, Max F., "Rapid Communication: Mapping of the Titin (TTN) Gene to Pig Chromosome 15" (1999). Animal Science Publications. 269.
https://lib.dr.iastate.edu/ans_pubs/269
Comments
This is an article from Journal of Animal Science 77 (1999): 2857, doi:/1999.77102857x. Posted with permission.