Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

2000

Journal or Book Title

Journal of Animal Science

Volume

78

Issue

12

First Page

3193

Last Page

3194

DOI

/2000.78123193x

Abstract

Locus Name. Vitamin D-25-hydroxylase. Species. Sus scrofa. Locus Symbol. CYP25. Source of Primers. PCR primers were designed from the published sequence for porcine vitamin D-25-hydroxylase (CYP25) cDNA (GenBank accession no. Y16417). The position of the forward and reverse primers corresponds to 506 to 530 bp and 830 to 854 bp, respectively, of the cDNA. The primer pair amplified a 348-bp fragment of the cDNA and an 862-bp fragment from the pig genomic DNA. The 862-bp fragment (Gen- Bank accession no. AF284378) was subcloned into a pNoTA vector and identity was confirmed by sequencing. Genomic sequences from 1 to 74, 169 to 329, and 749 to 862 showed 100% homology to the cDNA.

Comments

This is an article from Journal of Animal Science 78 (2000): 3193, doi:/2000.78123193x. Posted with permission.

Copyright Owner

American Society of Animal Science

Language

en

File Format

application/pdf

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