Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

2002

Journal or Book Title

Journal of Animal Science

Volume

80

Issue

5

First Page

1390

Last Page

1391

DOI

/2002.8051390x

Abstract

Source and Description of Primers. The conserved sequences of human and rabbit cholesteryl ester transfer protein gene (CETP) (GenBank accession numbers NM000078 and M27486, respectively) were used to design primers to PCR amplify porcine CETP. The primers amplified a 970-bp fragment of porcine CETP gene spanning an intron between exon 1 and exon 2. The porcine CETP sequence (GenBank accession number AF333037) determined from this fragment showed 80.9%(34/42) and 78.6% (33/42) nucleotide identity to the corresponding human and rabbit CETP exon 2 sequence, respectively. To physically map porcine CETP by PCR testing pig/ rodent somatic cell hybrid panel (SCHP) and to develop PCR-RFLP marker for linkage mapping in PigMaP reference families, a pig-specific reverse primer was designed based on the new generated porcine intron 1 sequence.

Comments

This is an article from Journal of Animal Science 80 (2002): 1390, doi:/2002.8051390x. Posted with permission.

Copyright Owner

American Society of Animal Science

Language

en

File Format

application/pdf

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