Campus Units
Animal Science
Document Type
Article
Publication Version
Published Version
Publication Date
2002
Journal or Book Title
Journal of Animal Science
Volume
80
Issue
5
First Page
1390
Last Page
1391
DOI
/2002.8051390x
Abstract
Source and Description of Primers. The conserved sequences of human and rabbit cholesteryl ester transfer protein gene (CETP) (GenBank accession numbers NM000078 and M27486, respectively) were used to design primers to PCR amplify porcine CETP. The primers amplified a 970-bp fragment of porcine CETP gene spanning an intron between exon 1 and exon 2. The porcine CETP sequence (GenBank accession number AF333037) determined from this fragment showed 80.9%(34/42) and 78.6% (33/42) nucleotide identity to the corresponding human and rabbit CETP exon 2 sequence, respectively. To physically map porcine CETP by PCR testing pig/ rodent somatic cell hybrid panel (SCHP) and to develop PCR-RFLP marker for linkage mapping in PigMaP reference families, a pig-specific reverse primer was designed based on the new generated porcine intron 1 sequence.
Copyright Owner
American Society of Animal Science
Copyright Date
2002
Language
en
File Format
application/pdf
Recommended Citation
Shi, X. W.; Zhang, Y. D.; Rothschild, Max F.; and Tuggle, Christopher K., "Rapid communication: Genetic linkage and physical mapping of the porcine cholesteryl ester transfer protein (CETP) gene" (2002). Animal Science Publications. 285.
https://lib.dr.iastate.edu/ans_pubs/285
Comments
This is an article from Journal of Animal Science 80 (2002): 1390, doi:/2002.8051390x. Posted with permission.