Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

1993

Journal or Book Title

Journal of Animal Science

Volume

71

Issue

12

First Page

3479

Last Page

3479

DOI

/1993.71123479x

Abstract

Source and Description of Probe. A 1,486-bp rat cDNA clone for myogenin (MYOG) was excised from the EcoRI site of the plasmid, pBluescrybe M13- (Wright et al., 1989). Method of Detection. DNA was isolated from whole blood and digested with MspI. Fragments were then separated by agarose gel electrophoresis and transferred to charged nylon membranes. Hybridizations were at 65°C for 16 to 20 h (.5 M NaCl, .05 M Naphosphate buffer, pH 6.5, 5x Denhardt's reagent, 10% dextran sulfate, .5% SDS, 100 pg/mL sonicated, denatured salmon sperm DNA). Final washes were at 55 to 60°C in .7x SSC, 5% SDS for 15 to 20 min.

Comments

This is an article from Journal of Animal Science 71 (1993): 3479, doi:/1993.71123479x. Posted with permission.

Copyright Owner

American Society of Animal Science

Language

en

File Format

application/pdf

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