Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

1994

Journal or Book Title

Journal of Animal Science

Volume

72

Issue

3

First Page

798

Last Page

798

DOI

/1994.723798x

Abstract

Source and Description of Probe. A 2.7-kb human cDNA clone for TAP2 was excised from the XbaI site of the plasmid pRSV.5neo (Bahram et al., 1991). Method of Detection. DNA was isolated from whole blood and digested with TuqI. Fragments were separated by agarose gel electrophoresis and transferred to charged nylon membranes. Hybridizations were for 16 to 20 h at 65°C (10% dextran sulfate, 7% SDS, .263 M Na2HP04, 1% BSA, 1 mM EDTA, 100 pglmL sonicated denatured salmon sperm DNA). Final washes were at 65°C in .7x SSC, 5% SDS for 15 to 20 min.

Comments

This is an article from Journal of Animal Science 72 (1994): 798, doi:/1994.723798x. Posted with permission.

Copyright Owner

American Society of Animal Science

Language

en

File Format

application/pdf

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