Journal or Book Title
Journal of Animal Science
Source and Description of Primers. We previously identified a SacI polymorphism by using a pig VCAM1 cDNA probe on Southern blots (VCAM1-1; Helm et al., 1994). This polymorphism was not informative enough to map VCAM1. To develop PCR-based genotyping, we sequenced the 3¢ untranslated region of pig VCAM1. Subsequently, a pig VCAM1 cDNA was deposited in Genbank; our data agree completely with that reported by Tsang et al. (Accession: U08351). The PCR primers were designed (forward, 5¢-TATCAGCCCTCCATAGTCACAT 3¢ and reverse, 5¢- GAAATTGTTGTCCATGACCTTTAT 3¢) .
American Society of Animal Science
Tuggle, Christopher K.; Yu, T. P.; Sun, H. S.; Wang, L.; and Rothschild, Max F., "Rapid Communication: Mapping the Pig VCAM1 Locus to Chromosome 4 Using a Double-Stranded Conformation Polymorphism Marker (VCAM1-2)" (1997). Animal Science Publications. 307.