Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

2015

Journal or Book Title

PloS ONE

Volume

10

Issue

11

First Page

e0143099

DOI

10.1371/journal.pone.0143099

Abstract

Heat stress and reduced feed intake negatively affect intestinal integrity and barrier function. Our objective was to compare ileum protein profiles of pigs subjected to 12 hours of HS, thermal neutral ad libitum feed intake, or pair-fed to heat stress feed intake under thermal neutral conditions (pair-fed thermal neutral). 2D-Differential In Gel Electrophoresis and gene expression were performed. Relative abundance of 281 and 138 spots differed due to heat stress, compared to thermal neutral and pair-fed thermal neutral pigs, respectively. However, only 20 proteins were different due to feed intake (thermal neutral versus pair-fed thermal neutral). Heat stress increased mRNA expression of heat shock proteins and protein abundance of heat shock proteins 27, 70, 90-α and β were also increased. Heat stress reduced ileum abundance of several metabolic enzymes, many of which are involved in the glycolytic or TCA pathways, indicating a change in metabolic priorities. Stress response enzymes peroxiredoxin-1 and peptidyl-prolyl cis-trans isomerase A were decreased in pair-fed thermal neutral and thermal neutral pigs compared to heat stress. Heat stress increased mRNA abundance markers of ileum hypoxia. Altogether, these data show that heat stress directly alters intestinal protein and mRNA profiles largely independent of reduced feed intake. These changes may be related to the reduced intestinal integrity associated with heat stress.

Comments

This article is published as Pearce, Sarah C., Steven M. Lonergan, Elisabeth Huff-Lonergan, Lance H. Baumgard, and Nicholas K. Gabler. "Acute heat stress and reduced nutrient intake alter intestinal proteomic profile and gene expression in pigs." PLoS One 10, no. 11 (2015): e0143099. doi: 10.1371/journal.pone.0143099. Posted with permission.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Copyright Owner

Pearce et al

Language

en

File Format

application/pdf

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