Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

2014

Journal or Book Title

Temperature

Volume

1

Issue

1

First Page

42

Last Page

50

DOI

10.4161/temp.28844

Abstract

Heat stress is associated with death and other maladaptions including muscle dysfunction and impaired growth across species. Despite this common observation, the molecular effects leading to these pathologic changes remain unclear. The purpose of this study was to determine the extent to which heat stress disrupted redox balance and initiated an inflammatory response in oxidative and glycolytic skeletal muscle. Female pigs (5–6/group) were subjected to thermoneutral (20 °C) or heat stress (35 °C) conditions for 1 or 3 days and the semitendinosus removed and dissected into red (STR) and white (STW) portions. After 1 day of heat stress, relative abundance of proteins modified by malondialdehyde, a measure of oxidative damage, was increased 2.5-fold (P < 0.05) compared with thermoneutral in the STR but not the STW, before returning to thermoneutral conditions following 3 days of heat stress. This corresponded with increased catalase and superoxide dismutase-1 gene expression (P < 0.05) and superoxide dismutase-1 protein abundance (P < 0.05) in the STR but not the STW. In the STR catalase and total superoxide dismutase activity were increased by ~30% and ~130%, respectively (P < 0.05), after 1 day of heat stress and returned to thermoneutral levels by day 3. One or 3 days of heat stress did not increase inflammatory signaling through the NF-κB pathway in the STR or STW. These data suggest that oxidative muscle is more susceptible to heat stress-mediated changes in redox balance than glycolytic muscle during chronic heat stress.

Comments

This article is published as Montilla, Sandra I. Rosado, Theresa P. Johnson, Sarah C. Pearce, Delphine Gardan-Salmon, Nicholas K. Gabler, Jason W. Ross, Robert P. Rhoads, Lance H. Baumgard, Steven M. Lonergan, and Joshua T. Selsby. "Heat stress causes oxidative stress but not inflammatory signaling in porcine skeletal muscle." Temperature 1, no. 1 (2014): 42-50. doi: 10.4161/temp.28844. Posted with permission.

Creative Commons License

Creative Commons Attribution-Noncommercial 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial 3.0 License

Copyright Owner

Landes Bioscience

Language

en

File Format

application/pdf

Share

COinS