Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

9-25-2018

Journal or Book Title

Scientific Reports

Volume

8

First Page

14336

DOI

10.1038/s41598-018-32710-w

Abstract

Arachidonic acid (C20:4) is related to a wide range of biological effects including lipid homeostasis. The fatty acid desaturase-2 (FADS2) gene encodes for the delta-6-desaturase, which is involved in the biosynthesis of C20:4 from linoleic acid (C18:2). The purpose of this study was to characterise mutations in the promoter of the porcine FADS2, evaluating in particular the effect of one haplotype tagging polymorphism (rs321384923A > G) on the biosynthesis pathway of C20:4. A total of 1,192 Duroc barrows with records on fatty acid composition in muscle and subcutaneous fat were genotyped. Pigs carrying the A allele showed, irrespective of fat content, both enhanced FADS2 expression and higher C20:4 in muscle and exhibited increased ratios of C20:4 to C18:2 and of C20:4 to eicosadienoic acid (C20:2) in both muscle and adipose tissue. Despite the inverse relationship observed between C20:4 and fat content, the rs321384923 polymorphism had no impact on lean weight. It is concluded that the haplotype encompassing the rs321384923 polymorphism at the porcine FADS2 affects the n-6 fatty acid profile by specifically modifying the desaturation efficiency of C18:2 to C20:4 rather than by concomitant variations in C18:2 following changes in fat content.

Comments

This article is published as Gol, Sofia, Ramona N. Pena, Max F. Rothschild, Marc Tor, and Joan Estany. "A polymorphism in the fatty acid desaturase-2 gene is associated with the arachidonic acid metabolism in pigs." Scientific reports 8 (2018): 14336. doi: 10.1038/s41598-018-32710-w.

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Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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The Authors

Language

en

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application/pdf

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