Campus Units
Animal Science
Document Type
Article
Publication Version
Published Version
Publication Date
2019
Journal or Book Title
Genes
Volume
10
Issue
7
First Page
546
DOI
10.3390/genes10070546
Abstract
Newcastle Disease (ND) is a continuing global threat to domestic poultry, especially in developing countries, where severe outbreaks of velogenic ND virus (NDV) often cause major economic losses to households. Local chickens are of great importance to rural family livelihoods through provision of high-quality protein. To investigate the genetic basis of host response to NDV, three popular Tanzanian chicken ecotypes (regional populations) were challenged with a lentogenic (vaccine) strain of NDV at 28 days of age. Various host response phenotypes, including anti-NDV antibody levels (pre-infection and 10 days post-infection, dpi), and viral load (2 and 6 dpi) were measured, in addition to growth rate. We estimated genetic parameters and conducted genome-wide association study analyses by genotyping 1399 chickens using the Affymetrix 600K chicken SNP chip. Estimates of heritability of the evaluated traits were moderate (0.18–0.35). Five quantitative trait loci (QTL) associated with growth and/or response to NDV were identified by single-SNP analyses, with some regions explaining ≥1% of genetic variance based on the Bayes-B method. Immune related genes, such as ETS1, TIRAP, and KIRREL3, were located in regions associated with viral load at 6 dpi. The moderate estimates of heritability and identified QTL indicate that NDV response traits may be improved through selective breeding of chickens to enhance increased NDV resistance and vaccine efficacy in Tanzanian local ecotypes.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright Owner
The Authors
Copyright Date
2019
Language
en
File Format
application/pdf
Recommended Citation
Walugembe, Muhammed; Mushi, James R.; Amuzu-Aweh, Esinam N.; Chiwanga, Gaspar H.; Msoffe, Peter L.; Wang, Ying; Saelao, Perot; Kelly, Terra; Gallardo, Rodrigo A.; Zhou, Huaijun; Lamont, Susan J.; Muhairwa, Amandus P.; and Dekkers, Jack C. M., "Genetic Analyses of Tanzanian Local Chicken Ecotypes Challenged with Newcastle Disease Virus" (2019). Animal Science Publications. 470.
https://lib.dr.iastate.edu/ans_pubs/470
Included in
Agriculture Commons, Genetics and Genomics Commons, Poultry or Avian Science Commons, Veterinary Preventive Medicine, Epidemiology, and Public Health Commons
Comments
This article is published as Walugembe, Muhammed, James R. Mushi, Esinam N. Amuzu-Aweh, Gaspar H. Chiwanga, Peter L. Msoffe, Ying Wang, Perot Saelao et al. "Genetic Analyses of Tanzanian Local Chicken Ecotypes Challenged with Newcastle Disease Virus." Genes 10, no. 7 (2019): 546. doi: 10.3390/genes10070546.