Campus Units

Animal Science

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

12-2013

Journal or Book Title

Reproductive Toxicology

Volume

42

First Page

68

Last Page

77

DOI

10.1016/j.reprotox.2013.07.026

Abstract

Insulin regulates ovarian phosphatidylinositol-3-kinase (PI3K) signaling, important for primordial follicle viability and growth activation. This study investigated diet-induced obesity impacts on: 1) insulin receptor (Insr) and insulin receptor substrate 1 (Irs1); 2) PI3K components (Kit ligand (Kitlg), kit (c-Kit), protein kinase B alpha (Akt1) and forkhead transcription factor subfamily 3 (Foxo3a)); 3) xenobiotic biotransformation (microsomal epoxide hydrolase (Ephx1), Cytochrome P450 isoform 2E1 (Cyp2e1), Glutathione S-transferase (Gst) isoforms mu (Gstm) and pi (Gstp)) and 4) microRNA’s 184, 205, 103 and 21 gene expression. INSR, GSTM and GSTP protein levels were also measured. Obese mouse ovaries had decreased Irs1, Foxo3a, Cyp2e1, MiR-103, and MiR-21 but increased Kitlg, Akt1, and miR-184 levels relative to lean littermates. These results support that diet-induced obesity potentially impairs ovarian function through aberrant gene expression.

Comments

This is a mansuscript of an article published as Nteeba, J., J. W. Ross, J. W. Perfield Ii, and A. F. Keating. "High fat diet induced obesity alters ovarian phosphatidylinositol-3 kinase signaling gene expression." Reproductive toxicology 42 (2013): 68-77. doi: 10.1016/j.reprotox.2013.07.026. Posted with permission.

Copyright Owner

Elsevier Inc.

Language

en

File Format

application/pdf

Published Version

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