Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

5-4-2017

Journal or Book Title

PloS ONE

Volume

12

Issue

5

First Page

e0176857

DOI

10.1371/journal.pone.0176857

Abstract

The food-borne pathogen Listeria (L.) monocytogenes is able to survive for months and even years in food production environments. Strains belonging to sequence type (ST)121 are particularly found to be abundant and to persist in food and food production environments. To elucidate genetic determinants characteristic for L. monocytogenes ST121, we sequenced the genomes of 14 ST121 strains and compared them with currently available L. monocytogenes ST121 genomes. In total, we analyzed 70 ST121 genomes deriving from 16 different countries, different years of isolation, and different origins—including food, animal and human ST121 isolates. All ST121 genomes show a high degree of conservation sharing at least 99.7% average nucleotide identity. The main differences between the strains were found in prophage content and prophage conservation. We also detected distinct highly conserved subtypes of prophages inserted at the same genomic locus. While some of the prophages showed more than 99.9% similarity between strains from different sources and years, other prophages showed a higher level of diversity. 81.4% of the strains harbored virtually identical plasmids. 97.1% of the ST121 strains contain a truncated internalin A (inlA) gene. Only one of the seven human ST121 isolates encodes a full-length inlA gene, illustrating the need of better understanding their survival and virulence mechanisms.

Comments

This article is published as Rychli, Kathrin, Eva M. Wagner, Luminita Ciolacu, Andreas Zaiser, Taurai Tasara, Martin Wagner, and Stephan Schmitz-Esser. "Comparative genomics of human and non-human Listeria monocytogenes sequence type 121 strains." PloS one 12, no. 5 (2017): e0176857. doi: 10.1371/journal.pone.0176857.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Copyright Owner

Rychli et al.

Language

en

File Format

application/pdf

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