Campus Units
Animal Science
Document Type
Article
Publication Version
Published Version
Publication Date
2017
Journal or Book Title
mSystems
Volume
2
Issue
6
First Page
e00141-17
DOI
10.1128/mSystems.00141-17
Abstract
Cytoplasmic incompatibility (CI) is an intriguing, widespread, symbiont-induced reproductive failure that decreases offspring production of arthropods through crossing incompatibility of infected males with uninfected females or with females infected with a distinct symbiont genotype. For years, the molecular mechanism of CI remained unknown. Recent genomic, proteomic, biochemical, and cell biological studies have contributed to understanding of CI in the alphaproteobacterium Wolbachia and implicate genes associated with the WO prophage. Besides a recently discovered additional lineage of alphaproteobacterial symbionts only moderately related to Wolbachia, Cardinium (Bacteroidetes) is the only other symbiont known to cause CI, and genomic evidence suggests that it has very little homology with Wolbachia and evolved this phenotype independently. Here, we present the first transcriptomic study of the CI Cardinium strain cEper1, in its natural host, Encarsia suzannae, to detect important CI candidates and genes involved in the insect-Cardinium symbiosis. Highly expressed transcripts included genes involved in manipulating ubiquitination, apoptosis, and host DNA. Female-biased genes encoding ribosomal proteins suggest an increase in general translational activity of Cardinium in female wasps. The results confirm previous genomic analyses that indicated that Wolbachia and Cardinium utilize different genes to induce CI, and transcriptome patterns further highlight expression of some common pathways that these bacteria use to interact with the host and potentially cause this enigmatic and fundamental manipulation of host reproduction.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Copyright Owner
Mann et al.
Copyright Date
2017
Language
en
File Format
application/pdf
Recommended Citation
Mann, Evelyn; Stouthamer, Corinne M.; Kelly, Suzanne E.; Dzieciol, Monika; Hunter, Martha S.; and Schmitz-Esser, Stephan, "Transcriptome Sequencing Reveals Novel Candidate Genes for Cardinium hertigii-Caused Cytoplasmic Incompatibility and Host-Cell Interaction" (2017). Animal Science Publications. 513.
https://lib.dr.iastate.edu/ans_pubs/513
Included in
Cell and Developmental Biology Commons, Entomology Commons, Molecular Genetics Commons, Parasitic Diseases Commons
Comments
This article is published as Mann, Evelyne, Corinne M. Stouthamer, Suzanne E. Kelly, Monika Dzieciol, Martha S. Hunter, and Stephan Schmitz-Esser. "Transcriptome sequencing reveals novel candidate genes for Cardinium hertigii-caused cytoplasmic incompatibility and host-cell interaction." MSystems 2, no. 6 (2017): e00141-17. doi: 10.1128/mSystems.00141-17.