Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

2-2019

Journal or Book Title

Plant Physiology

Volume

179

Issue

2

First Page

569

Last Page

587

DOI

10.1104/pp.18.01036

Abstract

Sucrose (Suc) is one of the most important types of sugars in plants, serving inter alia as a long-distance transport molecule, a carbon and energy storage compound, an osmotically active solute, and fuel for many anabolic reactions. Suc biosynthesis and degradation pathways are well known; however, the regulation of Suc intracellular distribution is poorly understood. In particular, the cellular function of chloroplast Suc reserves and the transporters involved in accumulating these substantial Suc levels remain uncharacterized. Here, we characterize the plastidic sugar transporter (pSuT) in Arabidopsis (Arabidopsis thaliana), which belongs to a subfamily of the monosaccharide transporter-like family. Transport analyses with yeast cells expressing a truncated, vacuole-targeted version of pSuT indicate that both glucose and Suc act as substrates, and nonaqueous fractionation supports a role for pSuT in Suc export from the chloroplast. The latter process is required for a correct transition from vegetative to reproductive growth and influences inflorescence architecture. Moreover, pSuT activity affects freezing-induced electrolyte release. These data further underline the central function of the chloroplast for plant development and the modulation of stress tolerance.

Comments

This article is published as Patzke, Kathrin, Pratiwi Prananingrum, Patrick AW Klemens, Oliver Trentmann, Cristina Martins Rodrigues, Isabel Keller, Alisdair R. Fernie et al. "The plastidic sugar transporter pSuT influences flowering and affects cold responses." Plant physiology 179, no. 2 (2019): 569-587. doi: 10.1104/pp.18.01036. Copyright American Society of Plant Biologists. Posted with permission.

Copyright Owner

American Society of Plant Biologists

Language

en

File Format

application/pdf

Available for download on Saturday, February 01, 2020

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