Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

5-8-2018

Journal or Book Title

Frontiers in Physiology

Volume

9

First Page

492

DOI

10.3389/fphys.2018.00492

Abstract

The Harderian gland is a sparsely characterized immune tissue known to play an important role in local immunity. The function of the Harderian gland, however, is not clearly defined. Measuring the expression of all genes using RNA-seq enables the identification of genes, pathways, or networks of interest. Our relative RNA-seq expression analysis compared the chicken Harderian gland transcriptome to other important primary and secondary immune tissues including the bursa of Fabricius, thymus, and spleen of non-challenged birds. A total of 2,386 transcripts were identified as highly expressed in the Harderian gland. Gene set enrichment showed the importance of G-protein coupled receptor signaling and several immune pathways. Among the genes highly expressed in the Harderian gland were 48 miRNAs, a category of genetic elements involved in regulation of gene expression. Several identified miRNAs have immune related functions. This analysis gives insight to the unique immune processes inherent in the Harderian gland.

Comments

This article is published as Deist, Melissa S., and Susan J. Lamont. "What makes the Harderian gland transcriptome different from other chicken immune tissues? A gene expression comparative analysis." Frontiers in Physiology 9 (2018): 492. DOI: 10.3389/fphys.2018.00492. Posted with permission.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Copyright Owner

Deist and Lamont

Language

en

File Format

application/pdf

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