Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

10-26-2018

Journal or Book Title

PLoS ONE

Volume

13

Issue

10

First Page

e0205824

DOI

10.1371/journal.pone.0205824

Abstract

Understanding biological response to stimuli requires identifying mechanisms that coordinate changes across pathways. One of the promises of multi-omics studies is achieving this level of insight by simultaneously identifying different levels of regulation. However, computational approaches to integrate multiple types of data are lacking. An effective systems biology approach would be one that uses statistical methods to detect signatures of relevant network motifs and then builds metabolic circuits from these components to model shifting regulatory dynamics. For example, transcriptome and metabolome data complement one another in terms of their ability to describe shifts in physiology. Here, we extend a previously described linear-modeling based method used to identify single nucleotide polymorphisms (SNPs) associated with metabolic changes. We apply this strategy to link changes in sulfur, amino acid and lipid production under heat stress by relating ratios of compounds to potential precursors and regulators. This approach provides integration of multi-omics data to link previously described, discrete units of regulation into functional pathways and identifies novel biology relevant to the heat stress response, in addition to generating hypotheses.

Comments

This article is published as Hubbard, Allen H., Xiaoke Zhang, Sara Jastrebski, Susan J. Lamont, Abhyudai Singh, and Carl J. Schmidt. "Identifying mechanisms of regulation to model carbon flux during heat stress and generate testable hypotheses." PLoS ONE 13, no. 10 (2018): e0205824. DOI: 10.1371/journal.pone.0205824. Posted with permission.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Copyright Owner

Hubbard et al.

Language

en

File Format

application/pdf

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