Campus Units
Animal Science
Document Type
Article
Publication Version
Published Version
Publication Date
11-27-2018
Journal or Book Title
Genes
Volume
9
Issue
12
First Page
579
DOI
10.3390/genes9120579
Abstract
Newcastle disease virus (NDV) is a devastating worldwide poultry pathogen with major implications for global food security. In this study, two highly inbred and genetically distinct chicken lines, Fayoumis and Leghorns, were exposed to a lentogenic strain of NDV, while under the effects of heat stress, in order to understand the genetic mechanisms of resistance during high ambient temperatures. Fayoumis, which are relatively more resistant to pathogens than Leghorns, had larger numbers of differentially expressed genes (DEGs) during the early stages of infection when compared to Leghorns and subsequently down-regulated their immune response at the latter stages to return to homeostasis. Leghorns had very few DEGs across all observed time points, with the majority of DEGs involved with metabolic and glucose-related functions. Proteomic analysis corroborates findings made within Leghorns, while also identifying interesting candidate genes missed by expression profiling. Poor correlation between changes observed in the proteomic and transcriptomic datasets highlights the potential importance of integrative approaches to understand the mechanisms of disease response. Overall, this study provides novel insights into global protein and expression profiles of these two genetic lines, and provides potential genetic targets involved with NDV resistance during heat stress in poultry.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright Owner
The Author(s)
Copyright Date
2018
Language
en
File Format
application/pdf
Recommended Citation
Saelao, Perot; Wang, Ying; Chanthavixay, Ganrea; Yu, Vivian; Gallardo, Rodrigo A.; Dekkers, Jack C. M.; Lamont, Susan J.; Kelly, Terra; and Zhou, Huaijun, "Integrated Proteomic and Transcriptomic Analysis of Differential Expression of Chicken Lung Tissue in Response to NDV Infection during Heat Stress" (2018). Animal Science Publications. 565.
https://lib.dr.iastate.edu/ans_pubs/565
Comments
This article is published as Saelao, Perot, Ying Wang, Ganrea Chanthavixay, Vivian Yu, Rodrigo A. Gallardo, Jack Dekkers, Susan J. Lamont, Terra Kelly, and Huaijun Zhou. "Integrated proteomic and transcriptomic analysis of differential expression of chicken lung tissue in response to NDV infection during heat stress." Genes 9, no. 12 (2018): 579. DOI: 10.3390/genes9120579. Posted with permission.