Campus Units
Animal Science, Statistics, Veterinary Microbiology and Preventive Medicine
Document Type
Article
Publication Version
Published Version
Publication Date
4-2016
Journal or Book Title
Developmental & Comparative Immunology
Volume
57
First Page
99
Last Page
106
DOI
10.1016/j.dci.2015.12.013
Abstract
Avian pathogenic Escherichia coli (APEC), an extraintestinal pathogenic E. coli (ExPEC), constitutes an animal health and a potential zoonotic risk. Most studies focus on the response of a single tissue to APEC infection. Understanding interactions among lymphoid tissues is of importance in controlling APEC infection. Therefore, we studied bone marrow, bursa, and thymus transcriptomes because of these tissues' crucial roles in development of pre-lymphocytes, B cells, and T cells, respectively. Using lesion scores of liver, pericardium, and air sacs, infected birds were classified as either resistant or susceptible. Little difference in gene expression was detected in resistant birds in bone marrow versus bursa or thymus, while there were large differences between tissues in susceptible birds. Phagosome, lysosome and cytokine interactions were strongly enhanced in thymus versus bone marrow in susceptible birds, and T cell receptor (TCR), cell cycle, and p53 signaling were significantly decreased. B cell receptor (BCR) was also significantly suppressed in bursa versus bone marrow in susceptible birds. This research provides novel insights into the complex developmental changes in gene expression occurring across the primary lymphoid organs and, therefore, serves as a foundation to understanding the cellular and molecular basis of host resistance to APEC infection.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Copyright Owner
The Authors
Copyright Date
2015
Language
en
File Format
application/pdf
Recommended Citation
Sun, Hongyan; Bi, Ran; Liu, Peng; Nolan, Lisa K.; and Lamont, Susan J., "Combined analysis of primary lymphoid tissues' transcriptomic response to extra-intestinal Escherichia coli (ExPEC) infection" (2016). Animal Science Publications. 582.
https://lib.dr.iastate.edu/ans_pubs/582
Comments
This article is published as Sun, Hongyan, Ran Bi, Peng Liu, Lisa K. Nolan, and Susan J. Lamont. "Combined analysis of primary lymphoid tissues' transcriptomic response to extra-intestinal Escherichia coli (ExPEC) infection." Developmental & Comparative Immunology 57 (2016): 99-106. DOI: 10.1016/j.dci.2015.12.013. Posted with permission.