Campus Units

Animal Science

Document Type

Article

Publication Version

Published Version

Publication Date

10-2016

Journal or Book Title

Developmental & Comparative Immunology

Volume

63

First Page

96

Last Page

110

DOI

10.1016/j.dci.2016.05.016

Abstract

Differences in responses of chicken bone marrow derived dendritic cells (BMDC) to in vitro treatment with lipopolysaccharide (LPS), heat, and LPS + heat were identified. The Fayoumi is more disease resistant and heat tolerant than the Leghorn line. Nitric Oxide (NO) production, phagocytic ability, MHC II surface expression and mRNA expression were measured. NO was induced in BMDC from both lines in response to LPS and LPS + heat stimulation; Fayoumi produced more NO with LPS treatment. Fayoumi had higher phagocytic ability and MHC II surface expression. Gene expression for the heat-related genes BAG3, HSP25, HSPA2, and HSPH1 was strongly induced with heat and few differences existed between lines. Expression for the immune-related genes CCL4, CCL5, CD40, GM-CSF, IFN-γ, IL-10, IL-12β, IL-1β, IL-6, IL-8, and iNOS was highly induced in response to LPS and different between lines. This research contributes to the sparse knowledge of genetic differences in chicken BMDC biology and function.

Comments

This article is published as Van Goor, Angelica, Anna Slawinska, Carl J. Schmidt, and Susan J. Lamont. "Distinct functional responses to stressors of bone marrow derived dendritic cells from diverse inbred chicken lines." Developmental & Comparative Immunology 63 (2016): 96-110. DOI: 10.1016/j.dci.2016.05.016. Posted with permission.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Copyright Owner

The Authors

Language

en

File Format

application/pdf

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